p jak Search Results


p-jak (tyr1007/1008) rabbit  (Wanleibio)
90
Wanleibio p-jak (tyr1007/1008) rabbit
P Jak (Tyr1007/1008) Rabbit, supplied by Wanleibio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p-jak (tyr1007/1008) rabbit/product/Wanleibio
Average 90 stars, based on 1 article reviews
p-jak (tyr1007/1008) rabbit - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
p–jak (af1486)  (Beyotime)
90
Beyotime p–jak (af1486)
Z734 downregulates the expression of ERK2 in MCF–7 cells. ( A ) MCF–7 cells were treated with Z734 (10 μM for 12 h). The expression levels of <t>p–JAK,</t> JAK, p–JNK, JNK, p–AKT, AKT, p–ERK, ERK, and GAPDH were determined via Western blotting. ( B ) Effects of Z734 on kinase activity <t>of</t> <t>ERK1,</t> ERK2, and MEK1. ( C ) Western blot analysis of the p–ERK2 and ERK2 expressions in MCF–7 cells exposed to 0, 5, 10, and 15 μM of Z734 for 12 h. Relative protein expression was normalized to GAPDH. ( D ) Western blot analysis of the p–ERK2 and ERK2 expressions in MCF–7 cells treated with Z734 (10 μM) for 0, 6, 12, and 24 h. ( E , F ) MCF–7 cells were exposed to Z734 (10 μM) for 12 h and were then stained with antibodies for ERK2 and p53. DAPI was used to stain the nuclei. Images were obtained using a fluorescence microscope. Quantification of fluorescence was performed on the average optical density using the ImageJ software. Scale bars = 10 μm. Data shown in ( E , F ) were used two–tailed Student’s t –test. All assays were performed in triplicate, and the data are expressed as mean ± standard deviation. ** p < 0.01.
P–Jak (Af1486), supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p–jak (af1486)/product/Beyotime
Average 90 stars, based on 1 article reviews
p–jak (af1486) - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
primary antibody against p-jak  (Elabscience Biotechnology)
90
Elabscience Biotechnology primary antibody against p-jak
Z734 downregulates the expression of ERK2 in MCF–7 cells. ( A ) MCF–7 cells were treated with Z734 (10 μM for 12 h). The expression levels of <t>p–JAK,</t> JAK, p–JNK, JNK, p–AKT, AKT, p–ERK, ERK, and GAPDH were determined via Western blotting. ( B ) Effects of Z734 on kinase activity <t>of</t> <t>ERK1,</t> ERK2, and MEK1. ( C ) Western blot analysis of the p–ERK2 and ERK2 expressions in MCF–7 cells exposed to 0, 5, 10, and 15 μM of Z734 for 12 h. Relative protein expression was normalized to GAPDH. ( D ) Western blot analysis of the p–ERK2 and ERK2 expressions in MCF–7 cells treated with Z734 (10 μM) for 0, 6, 12, and 24 h. ( E , F ) MCF–7 cells were exposed to Z734 (10 μM) for 12 h and were then stained with antibodies for ERK2 and p53. DAPI was used to stain the nuclei. Images were obtained using a fluorescence microscope. Quantification of fluorescence was performed on the average optical density using the ImageJ software. Scale bars = 10 μm. Data shown in ( E , F ) were used two–tailed Student’s t –test. All assays were performed in triplicate, and the data are expressed as mean ± standard deviation. ** p < 0.01.
Primary Antibody Against P Jak, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary antibody against p-jak/product/Elabscience Biotechnology
Average 90 stars, based on 1 article reviews
primary antibody against p-jak - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
p-jak abclone ap0772 antibody  (Abmart Inc)
90
Abmart Inc p-jak abclone ap0772 antibody
Z734 downregulates the expression of ERK2 in MCF–7 cells. ( A ) MCF–7 cells were treated with Z734 (10 μM for 12 h). The expression levels of <t>p–JAK,</t> JAK, p–JNK, JNK, p–AKT, AKT, p–ERK, ERK, and GAPDH were determined via Western blotting. ( B ) Effects of Z734 on kinase activity <t>of</t> <t>ERK1,</t> ERK2, and MEK1. ( C ) Western blot analysis of the p–ERK2 and ERK2 expressions in MCF–7 cells exposed to 0, 5, 10, and 15 μM of Z734 for 12 h. Relative protein expression was normalized to GAPDH. ( D ) Western blot analysis of the p–ERK2 and ERK2 expressions in MCF–7 cells treated with Z734 (10 μM) for 0, 6, 12, and 24 h. ( E , F ) MCF–7 cells were exposed to Z734 (10 μM) for 12 h and were then stained with antibodies for ERK2 and p53. DAPI was used to stain the nuclei. Images were obtained using a fluorescence microscope. Quantification of fluorescence was performed on the average optical density using the ImageJ software. Scale bars = 10 μm. Data shown in ( E , F ) were used two–tailed Student’s t –test. All assays were performed in triplicate, and the data are expressed as mean ± standard deviation. ** p < 0.01.
P Jak Abclone Ap0772 Antibody, supplied by Abmart Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p-jak abclone ap0772 antibody/product/Abmart Inc
Average 90 stars, based on 1 article reviews
p-jak abclone ap0772 antibody - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


Z734 downregulates the expression of ERK2 in MCF–7 cells. ( A ) MCF–7 cells were treated with Z734 (10 μM for 12 h). The expression levels of p–JAK, JAK, p–JNK, JNK, p–AKT, AKT, p–ERK, ERK, and GAPDH were determined via Western blotting. ( B ) Effects of Z734 on kinase activity of ERK1, ERK2, and MEK1. ( C ) Western blot analysis of the p–ERK2 and ERK2 expressions in MCF–7 cells exposed to 0, 5, 10, and 15 μM of Z734 for 12 h. Relative protein expression was normalized to GAPDH. ( D ) Western blot analysis of the p–ERK2 and ERK2 expressions in MCF–7 cells treated with Z734 (10 μM) for 0, 6, 12, and 24 h. ( E , F ) MCF–7 cells were exposed to Z734 (10 μM) for 12 h and were then stained with antibodies for ERK2 and p53. DAPI was used to stain the nuclei. Images were obtained using a fluorescence microscope. Quantification of fluorescence was performed on the average optical density using the ImageJ software. Scale bars = 10 μm. Data shown in ( E , F ) were used two–tailed Student’s t –test. All assays were performed in triplicate, and the data are expressed as mean ± standard deviation. ** p < 0.01.

Journal: Molecules

Article Title: A Novel ERK2 Degrader Z734 Induces Apoptosis of MCF–7 Cells via the HERC3/p53 Signaling Pathway

doi: 10.3390/molecules27144337

Figure Lengend Snippet: Z734 downregulates the expression of ERK2 in MCF–7 cells. ( A ) MCF–7 cells were treated with Z734 (10 μM for 12 h). The expression levels of p–JAK, JAK, p–JNK, JNK, p–AKT, AKT, p–ERK, ERK, and GAPDH were determined via Western blotting. ( B ) Effects of Z734 on kinase activity of ERK1, ERK2, and MEK1. ( C ) Western blot analysis of the p–ERK2 and ERK2 expressions in MCF–7 cells exposed to 0, 5, 10, and 15 μM of Z734 for 12 h. Relative protein expression was normalized to GAPDH. ( D ) Western blot analysis of the p–ERK2 and ERK2 expressions in MCF–7 cells treated with Z734 (10 μM) for 0, 6, 12, and 24 h. ( E , F ) MCF–7 cells were exposed to Z734 (10 μM) for 12 h and were then stained with antibodies for ERK2 and p53. DAPI was used to stain the nuclei. Images were obtained using a fluorescence microscope. Quantification of fluorescence was performed on the average optical density using the ImageJ software. Scale bars = 10 μm. Data shown in ( E , F ) were used two–tailed Student’s t –test. All assays were performed in triplicate, and the data are expressed as mean ± standard deviation. ** p < 0.01.

Article Snippet: The primary antibodies were diluted 1/500 and included Ki67 (AF173), p53 (AF7671), p–p53 (Ser15) (AF5893), p–p53 (Ser46) (AF5896), p–p53 (Thr55) (AF1696), BAX (AF0057), BCL–2 (AF0060), p–ERK1/2 (AM071), ERK1/2 (AF1051), ERK2 (AF1255), p–JAK (AF1486), JAK (AF1489), p–JNK (AJ516), JNK (AJ518), p–AKT (AF5734), AKT (AF0045), COX IV (AF6549), Histone H3 (AF0009), anti–GST (AF0174), anti–Flag (AF0036), anti–Myc (AF5054), anti–HA (AF0039) (Beyotime, Shanghai, China), p–p53 (Ser20) (ab157454) (Abcam, Wuhan, China), anti–ubiquitin (B0316), HERC3 (sc–100720) (Santa Cruz Biotechnology, Santa Cruz, CA, USA), and GAPDH (BL006B) (Biosharp, Wuhan, China).

Techniques: Expressing, Western Blot, Activity Assay, Staining, Fluorescence, Microscopy, Software, Two Tailed Test, Standard Deviation