Journal: Life

Article Title: Anticancer Effects of Weizmannia coagulans MZY531 Postbiotics in CT26 Colorectal Tumor-Bearing Mice by Regulating Apoptosis and Autophagy

doi: 10.3390/life14101334

Figure Lengend Snippet: Western blotting diagram of the effect of W. coagulans MZY531 postbiotics on the expression of apoptosis signaling pathway proteins. ( A ) Bax, Bcl2, and caspase-3 protein expressions in tumor tissue. ( B – D ) Bax, Bcl2, and caspase-3 protein expression gray value. ( E ) Phosphorylation of JAK2 and STAT3 and the expression levels of unphosphorylated JAK2 and STAT3 proteins in tumor tissue. ( F , G ) P-JAK2/JAK2 and P-STAT3/STAT3 protein expression gray value. Statistical analysis was conducted using ANOVA, with subsequent post hoc testing performed via Duncan’s multiple comparison test. Means with different superscript letters (a, b, c, and d) are statistically significantly different from each other ( p < 0.05), with ‘a’ representing the highest value and ‘d’ representing the lowest.

Article Snippet: Following blocking with 5% bovine serum albumin, the membranes were incubated with the appropriate antibodies: β-actin, p-JAK2, STAT3, Bcl-2 and Bax (GeneTex, San Antonio, TX, USA), JAK2, p-STAT3, PI3K, AKT, mTOR and p-mTOR (Bioss, Beijing, China), caspase-3 (Abcam, Cambridge, MA, USA), TGF-β, and SMAD4 (Proteintech, Wuhan, China).

Techniques: Western Blot, Expressing, Phospho-proteomics, Comparison